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Peptide GuidesMarch 1, 20268 min read

How to Reconstitute BPC-157: Complete Step-by-Step Guide

BPC-157 is one of the most popular healing peptides for injury recovery and tissue repair. This comprehensive guide covers everything you need to know about safely reconstituting and using BPC-157.

Medical Disclaimer

This guide is for educational purposes only. Always consult with a healthcare professional before using any peptide. BPC-157 is a research compound and not approved for human use by regulatory agencies.

What is BPC-157?

Body Protection Compound 157 (BPC-157) is a synthetic peptide derived from a protein found in gastric juice. It's gained popularity in the research community for its potential healing properties, particularly for:

  • Tendon and ligament repair: May accelerate healing of sports injuries
  • Muscle recovery: Potentially reduces recovery time after workouts
  • Gastrointestinal health: Originally studied for digestive tract healing
  • Wound healing: May promote faster skin and tissue repair

Common dosing ranges from 200-500 mcg daily, typically divided into 1-2 injections.

Materials You'll Need

Essential Supplies

  • BPC-157 vial (common sizes: 2mg, 5mg, 10mg)
  • Bacteriostatic water (BAC water) - sterile water with 0.9% benzyl alcohol
  • Insulin syringes - U-100 (1mL) with 29-31 gauge needles
  • Alcohol wipes - for sterilization
  • Mixing syringe - 3mL syringe for reconstitution

Optional but Recommended

  • Calculator or app - for precise dose calculations
  • Refrigerator - for proper storage
  • Sharps container - for safe needle disposal
  • Digital scale - to verify vial contents (advanced users)

Reconstitution Process

Step 1: Calculate Water Volume

The amount of bacteriostatic water you add determines your final concentration. Common ratios:

Example: 5mg BPC-157 Vial

  • 1mL water: 5,000 mcg/mL concentration (very strong)
  • 2mL water: 2,500 mcg/mL concentration (recommended)
  • 3mL water: 1,667 mcg/mL concentration (dilute)

💡 Pro Tip: Use our free peptide calculator to calculate the exact volumes and doses for your specific needs.

Step 2: Prepare Your Workspace

  • Clean your work surface thoroughly
  • Wash your hands and let them dry completely
  • Remove BPC-157 and BAC water from refrigerator
  • Allow both to reach room temperature (10-15 minutes)

Step 3: Draw the Water

  • Wipe the BAC water vial top with an alcohol wipe
  • Draw your calculated amount (e.g., 2mL) into the mixing syringe
  • Remove any air bubbles by gently tapping the syringe

Step 4: Add Water to Peptide

⚠️ Critical: Add water slowly to avoid damaging the peptide. Let the water run down the side of the vial, not directly onto the powder.

  • Wipe the BPC-157 vial top with alcohol
  • Insert the needle at a 45-degree angle against the glass wall
  • Inject water very slowly, letting it flow down the side
  • Do not shake or agitate the vial

Step 5: Mix Gently

  • Gently swirl the vial in circular motions
  • Allow 5-10 minutes for complete dissolution
  • The solution should be clear and colorless
  • If cloudiness persists, let it sit longer or gently swirl again

Dosing and Administration

Common Dosing Protocols

PurposeDaily DoseFrequencyDuration
General healing250-500 mcgOnce daily4-6 weeks
Acute injury250 mcgTwice daily2-4 weeks
Maintenance200 mcgOnce dailyOngoing

Injection Guidelines

  • Subcutaneous injection: Most common method, inject into fatty tissue
  • Local injection: Some inject near the injury site for targeted effects
  • Rotate injection sites: Avoid injecting in the same spot repeatedly
  • Common sites: Stomach, thigh, or upper arm fatty areas

Calculation Example

Example: 250 mcg dose from 5mg/2mL solution

  • Concentration: 5mg ÷ 2mL = 2,500 mcg/mL
  • Volume needed: 250 mcg ÷ 2,500 mcg/mL = 0.1mL
  • Syringe units: 0.1mL × 100 units/mL = 10 units on U-100 syringe

Storage and Safety

Storage Guidelines

  • Unreconstituted powder: Store in freezer (-18°C) for up to 2 years
  • Reconstituted solution: Store in refrigerator (2-8°C) for up to 30 days
  • Protect from light: Keep in original vial or wrap in aluminum foil
  • Avoid contamination: Always use sterile techniques

Safety Considerations

  • Source quality: Only purchase from reputable research chemical suppliers
  • Third-party testing: Look for certificates of analysis (COA)
  • Sterile technique: Always use alcohol wipes and sterile needles
  • Needle disposal: Use a sharps container for used needles
  • Monitor for reactions: Watch for redness, swelling, or allergic reactions

Common Mistakes to Avoid

❌ Don't Do This

  • Shake the vial vigorously
  • Use tap water instead of BAC water
  • Inject water directly onto the powder
  • Store at room temperature after mixing
  • Reuse needles or syringes
  • Skip alcohol sterilization

✅ Do This Instead

  • Gently swirl to mix
  • Always use bacteriostatic water
  • Let water flow down the vial wall
  • Refrigerate mixed solution immediately
  • Use fresh, sterile supplies each time
  • Sterilize everything with alcohol wipes

Ready to Calculate Your BPC-157 Doses?

Use our free peptide calculator to get exact measurements for your BPC-157 reconstitution. No guesswork, just precise calculations every time.

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